Good afternoon Emmanuel,

Could you please critque these Canine Ovulation Test Pads?  Are they reliable? http://www.aleshaneeaustralianshepherds.com/LH_Surge_Strips_For_.html 

Thank you!

Heather Dobson

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Hello Heather

Sorry for the delay, I was in Quebec last week and apparently missed this post! Anyway, let's try to answer this question!

First of all I never used these tests for ovulation detection. Apparently they are based on the semiquantitative evaluation (=colorimetric assay) of serum concentration of LH in the bitch. LH (Luteinizing Hormone) is secreted by the pituitary gland and peaks 48h before ovulation. When the day of LH peakis precisely known, it is then a very accurate technique for timing ovulation and scheduling the different breedings events.

Few comments however:

- LH is secreted in a pulsatile way. In research when we want to quantitatively detect the LH peak (= numerical measure like x ng/mL instead of evaluating a color change) we need to perform 2/3 blood samples per day until the LH peak is observed. Since it is a peak these blood samples need to be done daily (while with progesterone we don't need to be that strict). This has to be the same with this test I guess.

- I never heard about this specific test, Pfizer sold something similar calle Witness LH but there were publications related to its use, my former team in Paris assessed the efficacy of these LH kits to determine accurately the day of ovulation and had pretty interesting results (10/10 bitches pregnant just by using this test to detect ovulation). However concerning those Canine Ovulation Test Pads I never read anything concerning their use...

- From a personal point of view I would still prefer to use progesterone assays, or to combine it at least with progesterone assays so that ovulation can be confirmed. Typically if there is a LH surge, ovulation should occur but I saw many bitches doing anovulatory cycles despite levels of progesterone getting closed to ovulation values... We did not have access to LH assays at this time so it is hard to tell if the pattern of LH secretion was modified but at least using the two techniques make it more reliable, especially with a semiquantitative assay. If I had to use semiquantitative tests for timing of ovulation, I would rather use semiquantitative progesterone assays!

Hope this will help, let me know if you have more questions

Sincerely

Emmanuel Fontaine

PRO Technical Services

 

Thanks Emmanuel! :)

 

I was thinking of using the ovulation pads until LH surge.  Then the day LH surge is detected, do a progesterone test that same day (or following morning), and continue with progesterone testing until ovulation - as usual. 

 

With current progesterone testing, I do one more progesterone test after all breeding is complete, and of course would still do this.

 

Now just to get up the nerve to try these pads... but there's only one way to do that! LOL

 

Heather

 


 
Emmanuel PRO Technical Service said:

Hello Heather

Sorry for the delay, I was in Quebec last week and apparently missed this post! Anyway, let's try to answer this question!

First of all I never used these tests for ovulation detection. Apparently they are based on the semiquantitative evaluation (=colorimetric assay) of serum concentration of LH in the bitch. LH (Luteinizing Hormone) is secreted by the pituitary gland and peaks 48h before ovulation. When the day of LH peakis precisely known, it is then a very accurate technique for timing ovulation and scheduling the different breedings events.

Few comments however:

- LH is secreted in a pulsatile way. In research when we want to quantitatively detect the LH peak (= numerical measure like x ng/mL instead of evaluating a color change) we need to perform 2/3 blood samples per day until the LH peak is observed. Since it is a peak these blood samples need to be done daily (while with progesterone we don't need to be that strict). This has to be the same with this test I guess.

- I never heard about this specific test, Pfizer sold something similar calle Witness LH but there were publications related to its use, my former team in Paris assessed the efficacy of these LH kits to determine accurately the day of ovulation and had pretty interesting results (10/10 bitches pregnant just by using this test to detect ovulation). However concerning those Canine Ovulation Test Pads I never read anything concerning their use...

- From a personal point of view I would still prefer to use progesterone assays, or to combine it at least with progesterone assays so that ovulation can be confirmed. Typically if there is a LH surge, ovulation should occur but I saw many bitches doing anovulatory cycles despite levels of progesterone getting closed to ovulation values... We did not have access to LH assays at this time so it is hard to tell if the pattern of LH secretion was modified but at least using the two techniques make it more reliable, especially with a semiquantitative assay. If I had to use semiquantitative tests for timing of ovulation, I would rather use semiquantitative progesterone assays!

Hope this will help, let me know if you have more questions

Sincerely

Emmanuel Fontaine

PRO Technical Services

 

Let me know what you think of them, I received plenty of questions concerning their use and the answer I gave was always the same!!!!! Sure our PRO community will appreciate your feedback from the field!

Has anyone tried these yet?   What did you find with them?  Were they accurate?

I would be really interested in seeing how and by who these have been tried.  I have thought about using them myself,

but was a little nervous about trusting the results.

 

Any imput is appreciated.

Hello,

I'm interested in any results on this as well.

I haven't built up enough nerve...I'm afraid of missing the breeding all together.

Hi,

Just read the last comments on the thread. We'll touch on that during our last webinar, if you guys are interested there is a complete review of the different techniques that are available to determine the perfect breeding dates in dogs and what to expect from them (including the ovulation test pads) http://royalcaninbreedersclub.ning.com/profiles/blogs/timing-of-ovu...

Hope this will help,

Emmanuel

Hi folks.. It's Maureen from Canine Ovulation Test Pads.. There has been tremendous success with our customers using the OV pads as their only source of Progesterone Testing..  On a personal note... we have had three litters of 10-11-10 puppies only using the pads.. Customers are able to email a jpeg of their test pad and we can offer a second opinion of the test result..  If you are unsure and do Blood draw anyway, then run a side by side test.. many did and now longer do Blood Draw..but as with anything.. it is just another tool in your breeders tool kit...and very economical..Do whatever makes you feel comfortable.. Personally, we have saved a ton of money..but that is the nice thing about breeding... you are in total control of your breeding program.. All the best.. Maureen and Rod Paskin  

Thanks Maureen, your breeding success is very encouraging!

Can I get more information about the Ovulation Test Pads? 

Where to get them? How much they are? How to use them correctly? Etc. 

Thank you Jean 

Good Morning Jean..  

Not sure what info you are looking for but here is a recap for you, The strips turn colour from pink to purple/bluish when the LH surge is occurring..This gives you an indication that ovulation will occur within the next few days and that mating can be timed..  Here is the latest test study that I did personally in April 2016..  One of my bitches re-absorbed an entire litter last year (ultra sound at day 28 confirmed 6 puppies) but she could not keep her Progesterone levels high enough to support the pregnancy to term , so this year when she came in season, we had to do Blood draw progesterone testing to establish a firm number for comparison to track for the rest of the pregnancy,,  I used the strips to determine when she surged to cut down on the number of Blood draws we would need to do.. I knew that once the strips turned colour that it would take two days for the eggs to drop and two days for them to mature so decided to do the blood draw on the 3rd day after the strip turned colour.. We were right on.. She was 9.7 ng... perfect timing for a mating . We achieved a 20 minute natural tie and repeated the mating again the next day and that was all the matings we did..  Then on day 29 we did and ultra sound and found 9 or 10 heartbeats and viable puppies..   Four days after the ultra sound we did another Blood draw progesterone test to determine her progesterone levels and they had dropped to 8 ng, so under Vet care, she went on Regumate and on day 62, we did a C-section as she seemed in distress and we have nine beautiful puppies on the ground and not a single loss..  I mentioned the entire story because the blood draw numbers supported the strip findings and that is not the first time we have done side by side comparisons.. it just made sense to use this as a perfect example.. we have successfully  used the strips ourselves for 4 years and would not be without them at breeding time. You can purchase them at    http://aleshaneeaustralianshepherds.com/Canine_LH_Surge_Dete.html  . Let me know if you have any further questions.... info@aleshaneeaustralianshepherds.com.. Maureen 

By the way.. we loved the pads so much that we now have our own version which I have used for the last two years.. We own the LH Surge Detector Strips and have distributors worldwide..  They are one awesome little work horse

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